Tympanometry with low-frequency probe tone provided useful clinical information for patients with disorders of the tympanum, the tympanic membrane, and the Eustachian tube. Low-frequency, single component tympanometry, however, has a limitation to evaluate various middle ear lesions. Multiple frequency tympanometry can overcome it's limitation. A total 32 normal adults were tested with Vitual 310 impedance audiometry in 19 different frequency probe tones, ranging from 250 to 200 Hz. The resonance frequency, initial static compliance(initial Ytm), peak pressure(Pme, pressure at maximum compliance), static compliance in the peak pressure (Peak Ytm), and tympanometry width(T. width) calculated at 50% point of static compliance in the peak pressure were collected and analyzed to obtain their correlations with the change of frequencies. The resonance frequency of middle ear was most common in 1,000 Hz and all the resonance frequencies were within 2,000 Hz. The mean of initial Ytm, Pme, Peak Ytm, and T. width in each octave frequencies were as follows : 1) in 250 Hz probe tone, initial Ytm 1.684 mmho, Pme 10.703 daPa, Peak Ytm 2.751 mmho, T. width 85.5 daPa. 2) in 500 Hz probe tone, initial Ytm 2.231 mmho, Pme 10.922 daPa, Peak Ytm 3.947 mmho, T. width 69.1 daPa. 3) in 1000 Hz probe tone, initial Ytm 2.627 mmho. Pme 29.156 daPa, Peak Ytm 8.427 mmho, T. width 54.7 daPa. 4) in 2000 Hz probe tone, initial Ytm 4.356 mmho, Pme 67.649 daPa, Peak Ytm 7.341 mmho, T. width 54.352 daPa. Initial Ytm, Pme, Peak Ytm, and T. width were significantly increased when the probe tone frequencies were changed from 250 Hz up to 2000 Hz(p<0.005). The tympanometry width was significantly decreased in the increasing probe tone frequencies(p<0.005).