As the inner ear has very intricate and extensive vascular system, microcirc-ulation plays an essential role in maintaining inner ear homeostasis. Methods for the study on inner ear circulation can be divided into indirect and direct techniques. The laser Doppler flowmetry, the most recently introduced method for direct technique, means a dynamic measure of cochlear blood flow (CBF). It provides voltage output that is proportional to the number and velocity of red blood cells in a measurement volume. The purpose of this study was to define the method and characteristics of this technique in guinea pig. The results of this study were as follows ; 1) The data was relatively stable and reproducible. 2) The mean CBF of guinea pigs was 5.20±1.01ml/min/100g, and the mean blood pressure(BP) was 48.51±7.00mmHg under normal conditions. The value of data decreased as body temperature decreased. 3) The change pattern of CBF was similar to that of mean BP under asphyxia state, but the degree of change was larger than that of BP.