Detection of Human Papillomavirus by One Hour In Situ Hybridization in Laryngeal Papillomas |
Pan Soo Kim, MD, Jae Shik Cho, MD, Jung Jeong Rhyu, MD, Eun Ho Park, MD, and Chong Won Lee, MD |
Department of Otolaryngology-Head & Neck Surgery, College of Medicine, Chonnam University, Kwangju, Korea |
후두 유두종에서 One Hour In Situ Hybridization법과 면역조직화학적 방법에 의한 인형 유두종바이러스의 검출 |
김판수 · 조재식 · 류중정 · 박은호 · 이종원 |
전남대학교 의과대학 이비인후과학교실 |
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ABSTRACT |
The detection of human papillomavirus(HPV) DNA became to be performed rapidly and exactly owing to advancement of immunohistochemistry and moleculary biology. To investigate the infection site, detection rate and prevalent subtypes of HPV in 22 cases with laryngeal papilloma, HPV DNA was detected by one hour in situ hybridization(ISH). In immunohistochemical study, 3 of 8 cases were positive in juvenile type, and 3 of 14 cases in adult type were positive. Five of 16 cases were positive in male, and 1 of 6 cases was
positive in female. In one hour ISH. 19 cases(86.4%) were positive for HPV type 6/11 probe. Eleven cases(78.6%) of 14 cases in adult type were positive and all eight cases in juvenile type were positive for HPV type 6/11 probe. But all cases were negative for HPV type 16/18 and 31/33/35 probe. The one hour ISH has the advantage of demonstrating subtypes and location of infected cell by HPV without crushing of tissue and moreover it can be done more rapidly and safely with highly specificity and sensitivity than previous other ISH technique.
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Keywords:
In situ hybridizationㆍHuman papillomavirusㆍLaryngeal papillomaㆍImmunohistochemical study. |
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