| Experimental Study on the Pathogenesis of Otitis Media(Histopathological and Biochemical Characteristics of Middle Ear Mucosa Infected with Pseudomonas Aeruginosa in Rats) |
| Chong Sun Kim, MD1, Tai Kwon Cho, MD1, Il Tae Kang, MD1, Kwan Taik Noh, MD1, Woo-Hyun Chang, MD2, and Sun-Jun Choih, PhD3 |
| 1;Department of Otolaryngology, 2;Microbiology, College of Medicine, Seoul National University, 3;Department of Microbiology, Dental College, Seoul National University, Korea |
| 中耳炎의 病因에 關한 實驗的 硏究 |
| 金宗善1 · 趙泰權1 · 姜一泰1 · 盧寬澤1 · 張友鉉2 · 崔宣鎭3 |
| 서울大學校 醫科大學 耳鼻咽喉科學敎室1;微生物學敎室2;서울大學校 齒科大學 微生物學敎室3; |
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| ABSTRACT |
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Pathogenesis of otitis media was studied in an animal model of rats from a histopathological and biochemical point of view. Basic anatomical outline, and distribution and type of normal epithelial cells of the rat bulla were described as a beckground study. Pseudomonas otitis media was developed in rats by inoculating 109 bacteria into the tympanic bulla. Histopathologic change of the mucoperiosteal layer showed acute stage of infection from 3 days to 3 weeks, and it became chronic after 4 weeks animals through 12 weeks. Enzyme profile in the extracts of the inflammatory middle ear tissue was studied. The levels of three enzymes, PZ-peptidase, LDH, and lysozyme were much higher in the middle ear tissue than in the corresponding sera as might be expected. Tissue/serum ratios of the enzyme activities were 13-38 for PZ-peptidase, 63-177 for LDH, and 18-94 for lysozyme. Possible role of the PZ-peptidase and possible origins of the three enzymes detected in the tissue were discussed.
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