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Korean Journal of Otorhinolaryngology-Head and Neck Surgery > Volume 39(9); 1996 > Article
Korean Journal of Otorhinolaryngology-Head and Neck Surgery 1996;39(9): 1402-7.
Distribution of Tumor Necrosis Factor-α in Cholestratoma
Kwang Soo Kim, MD1, Cheol Kyu Park, MD1, Kyu Uang Whang, MD2, Byung Don Lee, MD1, Hyuck Soon Chang, MD1, and Ju Won Kang, MD1
1;Department of Otolaryngology & 2;Dermatology, College of Medicine, Soonchunhyang University, Seoul, Korea
중이 진주종 상피의 Tumor Necrosis Factor-α의 분포에 관한 연구
김광수1 · 박철규1 · 황규왕2 · 이병돈1 · 장혁순1 · 강주원1
순천향대학교 의과대학 이비인후과학교실1;피부과학교실2;

Chronic otitis media with cholestratoma is an inflammatory disease clinically characterized by bone destruction but the mechanisms of this destruction is not clearly known yet. There are various opinion about its mechanism. In recent years, various cytokines which secreted by activated macrophage are demonstrated by immunohistochemical method or enzyme-linked immuno-sorbent assay. These cytokines are tumor necrosis factor-α(TNF-α), interleukin-1α(IL-1α) and parathyroid hormone-related protein(PTHrP). The production of TNF-α by human macrophage in vitro was studied by many authors. We are interested in TNF-α. The epithelia of cholesteatoma tissue and normal external auditory canal skin were obtained from patients with cholesteatoma at ear surgery. Tissues were immediately frozen to -70 and 6 micrometer frozen sections were prepared and were stained by the indirect immunofluorescence method. Intensity of staining in cholesteatoma epitherium was more stronger than in nomal external auditory canal skin tissue. Cell culture was done. In the western blotting assay, a rather strong band was detected at choleateatoma epithelium. We firmly believe that the TNF-α of cholestratomatous keratinocytes is an important factor in the mechanism of bone destruction in cholesteatoma.

Keywords: TNF-αCholesteatomaImmunofluorescence stainWestern blot.
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