The papilloma is the most common benign tumor of the larynx, It is charaterized by its multiplicity and its tendency to recur. The laryngeal papilloma has been well known to be caused by human papillomavirus(HPV). The recurrent diseases are alleged to be associated with the latent infection of HPV. The HPV type 6 and 11 were found to be associated with the laryngeal papilloma, same as the genital condylomata. The DNA of HPV type 6 and 11 have been detected by several molecular biology techniques such as Southern blot hybridization and in-situ hybridization. Recently introduction of Polymerase Chain Reaction(PCR) enables more sensitive detection of these viral DNA by amplifying specific DNA sequence in a short time. PCR(polymerase chain reaction) was tried in order to detect the presence of HPV DNA in 15 cases of laryngeal papilloma. There were HPV 6 in 10 and HPV 11 in 6 out of 15 cases. Totally HPV DNA was detected in all cases of laryngeal pailloma.