In recent years many investigations are concentrated about aerospace medicine because it is the utmost important to have prolonged and repeated space trip as well as the far distance journey with various vehicle. In accordance with human flight in air-plane or space vehicle various changes in human and animal body, in response to positive acceleration, have naturally become the main problem to be solved. The author have attempted to find the effects of ratatory movement on albino-rat, particularly the changes of the serum protein. Serum protein and it's fractions were measured by the paper-electrophoresis on Grassmann and Hamming method. The experimental animals were utilized healthy albino-rat weighing 120-130 grams, and they were divided into the following main groups according to the various procedures. 1) Normal group. 2) Binding group. 3) Medication group (Chlorpromazine and Streptomycin hydrochloride). 4) Binding with medication group. 5) Rotation group (30 and 60 rotation). 6) Rotation group after binding and medication. After the various managements and preparations, the rats were sacrificed with interval of before procedure, immediate after procedure, 1,2,4,8 hours, 1,2,3,4 and 7 days. This study were resulted as follow ; 1) The total protein and distribution of protein fractions in normal group are showed as Tp : 6.5 gm/dl, Alb : 44.37%, α₁ : 9.06%, α₂ : 7.56%, β : 20.16% and γ : 19.02%. The normal ranges are showed also as Tp : 5.7-6.9 gm/dl, Alb : 40.94-47.42%, α₁ : 8.09-9.88%, α₂ : 6.06-8.92%, β : 18.84-21.56% and γ : 17.06-21.28%. 2) In binding, medication, and binding with medication group a slight changes were demonstrated throughout time but it was not significant values. 3) By the rotatory movement Alb and γ are influenced to be significant changes but Tp, α₁, α₂ and β fractions are not significant in changes. 4) In general the reaction of 60 rotation groups are demonstrated immediately after rotatory movement and two hours in 30 rotation groups. 5) Rotation group are demonstrated big change of the protein fraction as compared with rotation group after binding and chlorpromazine. 6) In changes of the Alb and γ fraction of rotation after binding and streptomycin was quite opposite results in comparing of results in rotation group and rotation group after binding and chlorpromazine. 7) The reaction of the Alb and γ fractions are depended upon the duration of rotatory movement and acceleration. 8) In this experimental study the changes of the protein fraction, as the result of the acceleration, was interpreted as secondary to a functional disturbance of the autonomic nervous system in the sympatheticotonic caused by labyrinthin stimulation.