The development of impedance audiometry during the past two decades has added new scope and dimension to clinical audiology. Based on the pioneering efforts of Metz, subsequent workers have refined instrumentation, technique, and interpretation to produce an invaluable tool for differential diagnosis. Particularly, there has been an increasing interest in using electroacoustic impedance measurement devices to assist in assessing the hearing sensitivity of young children and neonate. At present, a lack of information regarding impedance measurements in neonate limits the effective clinical use of this technique with this population. This paper describes measurements carried out using a Model TA-1D acoustic impedance bridge on a population of 70 neonates ranging in age from 25 to 142 hours. 1) Using Jerger's categories 112(93.3%) were type An tympanogram, 5(4.2%) were type Ad tympanogram, and 3 (2.5%) were type C tympanogram among 120 ears. An unexpected result of pressure-compliance testing was the observation of a double maximum or two distinct peaks near zero air pressure. The W pattern occurred in 3(2.5%) of the 120 ears. 2) Results of the compliance measurements indicated a mean of 0.72cc±0.20cc with a range from 0.25cc to 1.34cc. The sex difference was not expected but seemed to show a narrower compliance range in male neonates. There was little correlation between age and compliance in the first few hours of life. The actual distribution of ears in static compliance fits the prediction quite well and confirms the hypothesis that stable estimates of populations can be obtained from very small random samples of the population. 3) Measurement of the stapedial reflex was often obscured by a behavioral response to the pure tone stimuli. Of the 496 stimulus presentations, 309(62.3%) resulted in a clear stapedial reflex tested when contralateral stimuli were applied, while 70(14.1%) resulted in a behavioral response. No response was obtained on 117(23.6%) of the presentations. The mean threshold of stapedial reflex to pure tones was 106.1dB.