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Korean Journal of Otorhinolaryngology-Head and Neck Surgery 1995;38(7): 1042-8. |
Distribution of Sialoglycoconjugates and Their Penultimate Surgar in Developing Murine Olfactory and Respiratory Mucosa |
Jeung-Gweon Lee, MD, Joo-Heon Yoon, MD, Yoo Hyun Kim, MD, Kyung Su Kim, MD, Jin Woo Choi, MD, and Chang Hoon Kim, MD |
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea |
백서 후각점막 및 호흡점막에서 발육에 따른 씨알릭산의 분포 및 Neuraminidase 처치후의 변화 |
이정권 · 윤주헌 · 김유현 · 김경수 · 최진우 · 김창훈 |
연세대학교 의과대학 이비인후과학교실 |
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ABSTRACT |
The functional roles of sialoglycocojugates are to mediate cell adherence, to control the viscoelasticity of mucus and to serve as receptor sites for the binding of exogenous macromolecules such as those of bacterial or viral etiology. The purpose of this paper is to investigate the
distribution of sialoglycoconjugates and their penultimate sugar in developing murine nasal mucosa. Nasal cavities of mice(BALE/c) were fixed by intracardiac perfusion with 2.0% glutaraldehyde and embedded in Epon812. The serially sectioned slides were stained with Maackia amurensis(MAA) and Sambucus nigra(SNA). And then, the other slides were stained with Dolichos biflorus(DBA) and Arachis hypogaea(PNA) before and after neuraminidase treatment. In olfactory mucosa, all cell surfaces and Bowman's glands were labelled with MAA and
SNA from sixteeth gestational day. All cell swfaces were labelld with DBA after neuraminidase treatment. In respiraroty mucosa, all cell surfaces were labelled with SNA from the sixteenth gestational day. Cilia on a minority of ciliated cells in the mucosa of the septum and nasal floor were stained with MAA. Cell
surfaces of respiratory mucosa were labelled with PNA after neuraminidase digestion. However, goblet cells were
not labelled with MAA and SNA regardless of aging. Our results indicate that there are different carbohydrate structures of sialoglycoconjugates in the olfactory and respiratory mucosa.
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Keywords:
SialoglycoconjugatesㆍNasal mucosa. |
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