Autogenous fat has been used for correcting certain skin defects as well as for cosmetic enhancement. Recently the autogenous fat was thought to be a material for augmenting vocal cord palsy. However, there were few reports about voluminous and histological changes after autogenous fat transplantation. So we observed periodically the histological and voluminous loss of canine autogenous fat, which injected into iatrogenically paralyzed vocal cord and auricle. The fat cell injected into vocal cord showed minimal inflammatory change, fibrosis, foreign body reaction with relatively good viability. However, the fat cell injected into auricle showed absorption into surrounding tissue due to inflammatory change. We summarized that autogenous fat can be used for augmentation of the decreased tissue volume as substitution for Teflon or silicone in canine because there was minimal evidence of inflammatory change, foreign body reaction, fibrosis, diffusion of fat and etc. The only disadventage was fat atrophy. The injected fat volume into auricle was decreased nearly 70% compared with initial volume of fat. These results show that surgeon may have to over correct during injection or inject fat repeatedly to compensate this problem.