Recently, Yagi et al. developed a new phototubometric method which enables to detect tubal opening in both subjects with intact and perforated drum. We performed the Yagi's phototubometric recordings in 40 ears of 20 normal subjects for the standardization of phototubometric responses in normal subjects. In the 39 out of 40 ears, phototubometric responses were recorded at the external ear canal and no phototubometric recording was observed in the remaining one ear. In the 38 out of 39 ears, the phototubometric redordings showed upward shifting of waves and they were considered as positive waves that mean patent tube. One recordings showed only downward shifting of wave without upward shifting, and this wave was considered as a negative wave for the tubal patency. One ear, did not show any phototubometric response, is also considered as a negative wave.