One of the most difficult and challenging problems in head and neck field is the management of laryngeal of tracheal defects secondary to stenosis or malacia. Moreover the problem is increasing with greater number of surviving patients, after prolonged ventilating assistance, particularly the neonate and the elderly. End to end anastomosis after resection of 6cm of the trachea is the method of choice, in most patients. However, the reconstruction of more than half the trachea according to biological principles makes it desirable to use autogenous tissue meeting the requirement of reliability, stability, and compatibility. Authors have used the rib cartilage and perichondrium as the graft material because cartilage is the normal supporting matrix of trachea, to investigate whether they will be of obvious advantage for positioning graft over the defect and obtaining new cartilage there. Through the experiment, we confirmed rib cartilage and perichondrium as a suitable material for the reconstruction of the tracheal defects.